Fig. 2.

HBMSC-Exos promotes autophagy in OS cells. (A) HOS and MG63 cells transfected with GFP-mRFP-LC3 lentivirus were cultured with PBS and hBMSC-Exos. Yellow and red puncta were observed and counted under confocal microscopy (n = 3). (B) Western blot analysis of autophagy-related protein levels, including Beclin1, p62, ATG5, and LC3-II in HOS and MG63 cells (n = 3). (C) TEM was used to detect the autophagic microstructure of HOS and MG63 cells after administering PBS and hBMSC-Exos. Red arrows indicate a cellular autophagosome with a double-layer structure or an autolysosome generated by the fusion of an autophagosome and lysosome. *P < 0.05, ^⁎⁎P < 0.01, ^⁎⁎⁎P < 0.001.