Fig 4.

CQ combined with zinc enhanced the radiosensitivity of CNE-2s cells. (A) Representative crystal violet staining photos of CNE-2s cells irradiated with 2 Gy and 10 Gy. All values shown were mean ± SD of triplicate measurements and repeated three times with similar results. Clone formation assay was used to detect the radiosensitivity of CNE-2s cells with different treatments. (B) Representative images of acridine orange staining in CNE-2s. (C) Representative images of GFP-LC3 tracer protein. (D) Representative images of transmission electron microscopy to observe the structure and number of autophagosomes. (E) Western blotting analysis of Beclin1, LC3A and LC3B protein levels in CNE-2s cells.