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. 2020 Jan 14;16(5):739–751. doi: 10.7150/ijbs.40516

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TASP1 promotes GBC cell proliferation and tumor growth. (A) mRNA expression of TASP1 in the HIBEC and GBC cell lines including NOZ, SGC-996, EH-GB-1 and GBC-SD. (B-C) mRNA and protein expression levels of TASP1 were examined in GBC-SD and EH-GB-1 cells transfected with Lv-shTASP1. (D) The proliferation of treated GBC cells was measured by CCK-8 assay. (E) The colony formation was assessed in GBC cells and statistical significance was analyzed based on the numbers of colonies. (F) The tumor size and weight were measured, which were formed in nude mice injected with the Lv-shNC- and Lv-shTASP1-GBC-SD cells. (G) The expression levels of TASP1 and Ki67 were detected in GBC subcutaneous xenograft model by IHC (scale bar, 50 μm). All results (mean ± SD) are from three separate experiments; **P < 0.01, ***P < 0.001.