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. 2020 Jan 14;16(5):739–751. doi: 10.7150/ijbs.40516

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FAM49B promotes GBC cell proliferation and metastasis. (A) The mRNA expression level of FAM49B was downregulated in GBC-SD and EH-GB-1 cells transfected with Lv-shFAM49B. (B-C) Cell proliferation and colony formation abilities were inhibited in GBC-SD and EH-GB-1 cells transfected with Lv-shFAM49B. (D) Knockdown of FAM49B suppressed the migration and invasion abilities of the GBC-SD and EH-GB-1 cells. (E-F) Statistical significance of colony formation and metastasis assays was analyzed according to the number of colony and invaded cells; **P < 0.01, ***P < 0.001.