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. 2020 Jan 16;10(5):2385–2398. doi: 10.7150/thno.41708

Figure 2.

Figure 2

(A) SKOV-3 cells were treated with TPD@TB/KBU2046 (10 μg/ml), PD@TB/KBU2046 (10 μg/ml), TPD@TB (10 μg/ml), TPD@KBU2046 (10 μg/ml) and TPD (10 μg/ml), respectively, and then irradiated with white light (100 mW cm-2, 3 min). The ROS production in SKOV-3 cells was detected by DCFH-DA assay. Green fluorescence (DCF, Ex =488 nm, Em=505-540 nm); Red fluorescence (TPD@TB/KBU2046, PD@TB/KBU2046, TPD@TB, Ex: 488 nm, Em: 620-720 nm). Scale bar: 10 μm. (B) SKOV-3 cells were cultured with TPD@TB/KBU2046 (10 μg/ml), PD@TB/KBU2046 (10 μg/ml), TPD@TB (10 μg/ml), TPD@KBU2046 (10 μg/ml) and TPD (10 μg/ml), respectively, and then irradiated with white light (100 mW cm-2, 3 min), respectively. The dead SKOV-3 cells were detected by PI staining. Red fluorescence (PI, Ex: 543 nm, Em: 620-680 nm). Scale bar: 100 μm. (C) SKOV-3 cells were incubated with different concentrations of TPD@TB/KBU2046, PD@TB/KBU2046, TPD@TB, TPD@KBU2046 and TPD nanoparticles and irradiated with white light (100 mW cm-2, 3 min), respectively. Then the viability of the SKOV-3 cells was evaluated by CCK-8 assay. Data were represented as mean ± SD (n =3). The results were analyzed via two-sided Student's t-test. ** P <0.01, n.s., not significant. (D) Expression of PCNA in SKOV-3 cells treated with TPD@TB/KBU2046, PD@TB/KBU2046, TPD@TB, TPD@KBU2046 and TPD upon light irradiation (white light, 100 mW cm-2, 3 min).