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. 2020 Jan 12;10(5):2188–2200. doi: 10.7150/thno.39151

Figure 2.

Figure 2

Acetylation has no effects on the reductase activity of AKR1C1. (A) Bacterially expressed GST-AKR1C1-2KR protein had a dramatically decreased acetylation level compared with GST-AKR1C1-WT. (B-C)Michaelis-Menten Plots for S-tetralol catalyzed by AKR1C1-WT and AKR1C1-2KR, each point represents the mean ± SD of at least three experiments. (D)Kinetic parameters for S-tetralol catalyzed by AKR1C1-WT and AKR1C1-2KR were acquired from 100 mM sodium phosphate buffer, at PH7.0, with 200 μM NADP+. (E) The position of lysine185/201 in the AKR1C1-NADP+-3-chloro-5-phenylsalicylic acid (CPSA) complex (RCSB PDB: 3NTY). The coenzyme NADP+ and inhibitor CPSA that interact with AKR1C1 are depicted using stick models.