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. 2020 Jan 12;10(5):2141–2157. doi: 10.7150/thno.38704

Figure 6.

Figure 6

UQCRC1 knockdown suppresses PDAC cell growth by inhibiting mitochondrial OXPHOS and ATP generation. (A) Relative cell growth in UQCRC1 knockdown PANC-1 and CFPAC-1 cells as measured by CCK8 (n = 4, sc: scramble, two-way ANOVA). (B) Representative images and quantitative analysis of the declined colony formation ability of UQCRC1 knockdown PANC-1 and CFPAC-1 cells (n = 5). (C) UQCRC1 knockdown caused G0/G1 phase accumulation (t-test for G0/G1 phase and P < 0.001). (D) Relative cell growth of PANC-1 and CFPAC-1 cells stably expressing shUQCRC1 in the presence or absence of 10 nM ATP for 48 h (n = 4, two-way ANOVA). (E) Ratio of NAD+/NADH in PANC-1 and CFPAC-1 cells with or without UQCRC1 downregulation (n = 4). (F) Ratio of ADP/ATP in PANC-1 and CFPAC-1 cells with or without UQCRC1 downregulation (n = 4). (G) Intracellular ATP and eATP content of PANC-1 and CFPAC-1 cells with or without UQCRC1 downregulation. (H) Decreased OCRs in PANC-1 and CFPAC-1 cells with UQCRC1 downregulation (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.