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. 2020 Jan 12;10(5):2243–2259. doi: 10.7150/thno.35861

Figure 3.

Figure 3

ZNF471 inhibits ESCC cell migration and invasion and EMT. (A) Morphological changes of ESCC cells infected with control vector and ZNF471 by phase contrast microscopy. Scale bars: 200 μm. (B) Cell migration abilities of ESCC cells evaluated by wound healing assays. Photographs captured at 0, 12 and 24 h. The cellular migration (C) and invasion (D) abilities of ESCC cells upon ectopic expression of ZNF471 were measured by Transwell assays with or without Matrigel. Representative images were photographed following fixation and staining. Scale bars: 50 μm. (E, F) qRT-PCR and western blot analysis of EMT, and downstream target markers. Student's test was used. Data are presented as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001.