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. 2020 Jan 12;10(5):2243–2259. doi: 10.7150/thno.35861

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Knockdown of ZNF471 promotes ESCC cell growth and induces metastasis. (A, B) Evaluation of ZNF471 knockdown in KYSE270 cells after transfection with ZNF471 siRNA and si-NC by RT-PCR and western blotting. (C) The effect of ZNF471 knockdown on cell viability was measured with cell viability assays. (D, E) Cell cycle distribution after ZNF471 knockdown in KYSE270 cells. (F) Transwell assays showed cell migration and invasion of KYSE270 cells after ZNF471 knockdown. Scale bars: 50 μm. Each experiment was repeated three times. Student's test was used. Data are presented as the mean ± SD.*p<0.05, **p<0.01, ***p<0.001.