Figure 4.

Activated NOTCH4 (NICD4) induced an ML-BCSC expression profile. (A-D) GSEA analysis of RNA-seq data of NICD4 overexpressing SUM149 exhibited enrichment of (A) stemness regulating genes, (B) mesenchymal-like transition associated genes, (C) invasion enhancing genes and (D) cell cycle arrest associated genes. (E) GO analysis of the RNA-seq data indicated enriched motility associated genes. (F) qRT-PCR was performed to validate the significantly changed genes associated with cell cycle arrest and EMT related genes in 3 TNBC cell lines overexpressing NICD4, including SUM149, MDA-MB-231 and SUM159. The RNA-seq results of SUM149 were also presented in the heatmap for comparison. TBP was used as internal control. Data was shown as log₂ of fold change relative to the control group of each cell line.