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. 2020 Feb 7;8:46. doi: 10.3389/fcell.2020.00046

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Copyright © 2020 Imai and Takahashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phagocytosis and chemotaxis of hemocytes (granulocytes) from Marsupenaeus japonicus. (A) Granulocyte phagocytizing yeasts. Yeast is indicated by the arrow. A Giemsa-stained sample is shown after 2 h incubation with heat-killed yeast on the glass. Scale bar: 10 μm. (B) A migrated granulocyte. Chemoattractant was placed on the bottom compartment, and granulocytes were added to the upper compartment and incubated for 4 h at 24°C in the Boyden chamber with PVP membrane. The membrane was fixed with 20% formalin and stained with Giemsa solution. A migrated granulocyte is indicated by a black arrow (lower surface of the membrane), and a pre-migrated granulocyte is indicated by a white arrow (upper surface of the membrane). Scale bar: 5 μm, pore size: 5 μm.