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. 2020 Feb 7;8:59. doi: 10.3389/fbioe.2020.00059

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Copyright © 2020 Rodrigues, Gomes and Rodrigues.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production of curcumin from ferulic acid in E. coli BL21 (DE3) using different plasmids and conditions, after 63 h of fermentation. pRSFduet_CURS1 and pCDFduet_DCS were combined with pAC_4CL (1,3) or pACYCDuet_4CL (2,4). Ferulic acid was added at a concentration of 2 mM at time 0 of induction in M9 (1–4) and 1 mM of ferulic acid was also added after 15 h (3,4). 4CL, 4-coumarate-CoA ligase; DCS, diketide-CoA synthase; CURS1, curcumin synthase 1.