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. 2020 Feb 7;8:59. doi: 10.3389/fbioe.2020.00059

FIGURE 7.

FIGURE 7

Curcuminoids production in co-culture experiments, after 63 h of fermentation. Each E. coli BL21 (DE3) carried a module of the pathway. The difference between the two co-cultures was the first module. Co-culture 1 contained one E. coli carrying pCDFduet_TAL, pRSFduet_C3H and pACYCduet_COMT, and co-culture 2 one E. coli carrying pRSFduet_TAL, pCDFduet_C3H and pACYCduet_COMT. The second module of the pathway was common to both co-cultures and was composed of pACYCduet_4CL, pCDFduet_DCS and pRSFduet_CURS1. TAL, tyrosine ammonia lyase; C3H, 4-coumarate 3-hydroxylase; COMT, caffeic acid O-methyltransferase; 4CL, 4-coumarate-CoA ligase; DCS, diketide-CoA synthase; CURS1, curcumin synthase 1.