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. 2020 Jan 10;12(1):196. doi: 10.3390/nu12010196

Figure 3.

Figure 3

Effect of GJ/CDDP combination on apoptosis factors in U87MG and U373MG cells. (a) Western blot assays on cleaved caspase-3, cleaved caspase-9, and cleaved PARP were performed in GJ/CDDP combination-treated U87MG cells. (b) Annexin V assay was performed in GJ/CDDP combination-treated U87MG cells. (c) An MTT assay was performed in GJ/CDDP combination-treated U87MG cells after pre-treatment with Z-VAD-FMK. (d) Western blot assays on cleaved caspase-3, cleaved caspase-9, and cleaved PARP were performed in GJ/CDDP combination-treated U373MG cells. (e,f) An MTT assay was performed in GJ/CDDP combination-treated U373MG cells after pre-treatment with Z-VAD-FMK. Results are displayed as mean ± S.E. of three or more separate experiments. GAPDH was used as endogenous control. *** p < 0.005 vs. untreated cells. GJ, Gardenia jasminoides; CDDP, cisplatin.