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. 2020 Jan 10;12(1):196. doi: 10.3390/nu12010196

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of GJ/CDDP combination under autophagy-inhibited conditions in U87MG and U373MG cells. (a) A Western blot assay on LC3B-I/II and (b) an MTT assay was performed in GJ/CDDP combination-treated U87MG cells after pre-treatment with 3-MA. (c) A Western blot assay on LC3B-I/II and (d) an MTT assay was performed in GJ/CDDP combination-treated U87MG cells after pre-treatment with CQ. (e) A Western blot assay on LC3B-I/II and (f) an MTT assay was performed in GJ/CDDP combination-treated U373MG cells after pre-treatment with 3-MA. (g) A Western blot assay on LC3B-I/II and (h) an MTT assay was performed in GJ/CDDP combination-treated U373MG cells after pre-treatment with CQ. Results are displayed as mean ± S.E. of three or more separate experiments. GAPDH was used as endogenous control. *** p < 0.005 vs. untreated cells. GJ, Gardenia jasminoides; CDDP, cisplatin.