Table 1.

Sensitivity of the single and multiplex assays.

Specificity	Fluorescence Dye	Singleplex Assay			Multiplex Assay
		Limit of Detection	Mean ct Value	SD	Limit of Detection	Mean ct Value	SD
Clade 2.3.4.4	Hex	5 × 101	34.39	0.025	5 × 101	33.84	0.03
Clade2.3.2.1	FAM	5 × 101	33.83	0.020	5 × 101	34.5	0.025
Pan-H5 HPAI	Texas red	5 × 101	34.12	0.061	5 × 101	33.89	0.06

Each concentration had triple replicates and the mean and SD were conducted using GraphPad Prism software (version 5.1).