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. 2020 Jan 14;9(1):224. doi: 10.3390/jcm9010224

Figure 2.

Figure 2

Spermatogonial expression of PCNA and 5mC throughout age. (A) Immunohistochemical identification of PCNA-positive (brown) spermatogonia at several testicular developmental stages. Scale bar: 20 µm. (B) Density and labeling index (%) of PCNA-positive spermatogonia during normal development (NT group) and adulthood. (C) Immunohistochemical identification of 5mC-positive (brown) spermatogonia at several testicular developmental stages. Scale bar: 20 µm. (D) Density and labeling index (%) of 5mC-positive spermatogonia during normal development (NT group) and adulthood. Individual values, trend line, and 95% confidence interval are represented. Generalized linear models were used to predict the response of labeling index based on the patients’ age. Statistically significant difference is represented as * p < 0.05.