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TLR3 and MDA5 play important immunity roles in DTMUV-infected DEF cells. DEF cells were infected with or without DTMUV and harvested at 12, 24, 36, 48, and 60 hpi. The qRT-PCR analysis was performed to examine the mRNA expression levels of RLRs (D), and Toll-like receptors (TLRs) (A–C). Comparison of TLRs and RLRs in DEF cells (E,F). The average levels from three independent experiments are plotted. The error bars represent the SEs. * P < 0.05, ** P < 0.01.
