Skip to main content
. 2020 Jan 15;12(1):105. doi: 10.3390/v12010105

Figure 4.

Figure 4

EBOV NP-R111C increases budding of VLPs. (A) Schematic of the VLP budding assay. We expressed NLuc fused to EBOV VP40 (NLuc-VP40) in HEK 293FT cells to form luminescent VLPs, and co-expressed NP mutants to measure the impact of NP genotype on VLP budding; (B) VLP budding assay control. NLuc-VP40 expression alone resulted in bright luminescence, expressed in relative light units (RLU). VP40 loss-of-function (LOF) mutant L117R failed to form VLPs (n = 6 biological replicates). We assessed statistical significance by paired t-test. Error bars indicate mean ± standard error of the mean (SEM); (C) VLP budding with NP variants. We measured and normalized all NP or eGFP RLU values within each replicate to that replicate’s NP-R111 RLU (n = 6 biological replicates) and assessed statistical significance using repeated measures ANOVA with Dunnett’s test to correct for multiple hypothesis testing. Error bars indicate mean ± SEM.