Table 1.
Antibodies/Dye | Host | Dilution | Supplier | Cat/Lot number |
---|---|---|---|---|
Primary | ||||
Cleaved Caspase 3 (Asp175) | Rabbit | 1:250 | Cell Signaling | 9661S |
Cytokeratin 20 (CK20) (SP33) | Rabbit | 1:1 | Roche Ventana | 790-4431 |
Caudal type homeobox 2 (CDX2) | Rabbit | 1:1000 | Abcam | Ab76541 |
Leucine rich repeat containing G protein-coupled receptor 5 (LGR5) | Rabbit | 1:200 | Genentech | n/a |
Secondary | ||||
Anti-rabbit horseradish peroxidase | Goat | 1:200 | Life Technologies | G21234 |