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. 2019 Dec 19;9(1):9. doi: 10.3390/jcm9010009

Figure 5.

Figure 5

Immunofunction of hnRNPA2/B1 in PBMCs of AED patients and in f-LSCs. (A) PBMCs collected from AED patients were activated with anti-CD3 mAb and incubated with anti-PD-1 human recombinant protein or f-LSCs. After anti-PD-1 blocking, the molecular detection of IL-2, Bcl-2, PDL-1, p27Kit, CCND1and IFN-γ mRNA by qRT-PCR was assessed. (B) hnRNPA2/B1 siRNA knowdown in activated PBMCs of AED patients is shown. At 72 h post electroporation, expression for FOXP3, TGF-β, IL-17A, Arg-1 and IL-4 was detected. (C) Expression levels of several immunosuppressive and tolerogenic markers (TGF-β, IDO, COX-2, HGF, PDL-1/2, FAS, IL-6) in f-LSCs after chemical transient silencing of hnRNPA2/B1 protein are reported. All results are shown as mean ± SD of five independent experiments, * p < 0.05, ** p < 0.02, *** p < 0.001.