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. 2019 Dec 19;9(1):9. doi: 10.3390/jcm9010009

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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hnRNPA2/B1 mRNA levels are influenced by NF-ĸB transcription factor activity in Jurkat T cells. (A) Jurkat cells were activated for 5 hours with 10 µg of PHA and treated with 10 µM of parthenolide to induce or block the NF-kB nuclear action respectively. Unstimulated cells were used as negative controls. All results are shown as mean ± SD of five independent experiments, ** p < 0.02, *** p < 0.001. (B) Protein extracts prepared from Jurkat cells were immunoprecipitated with human anti-hnRNPA2/B1 antibody. The immune complexes and the input (50 µg of total extracts used in the immunoprecipitation) were analysed by immunoblotting with antibodies specific to human p65-RelA and hnRNPA2/B1. M: marker; IP: immunoprecipitated.