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Detection of PERV-A/C proviruses in GöMP from the Göttingen University. PBMCs from three consecutive bleedings (bleedings 2, 3 and 4) were isolated and tested at day 0 and after 5 days of cultivation with PHA. Two primer pairs were used resulting in overlapping amplicons (Figure 2). 11 pigs (A–K) were analyzed, as well as a PERV-A/C plasmid PERV-A 14/220 ([37], AY570980) (P) and as negative control (N) water.
