Table 2.
Primers and probes used for virus screening.
| Primers Used | Sequence 5′-3′ | Nucleotide Position | Accession Number | Reference |
|---|---|---|---|---|
| Primers used for PCR | ||||
| PLHV-1,-2 (747) fw PLHV-1,-2 (747) rev |
CAYGGTAGTATTTATTCAGACA GATATCCTGGTACATTGGAAAG |
21,146–21,167 21,488–21,467 |
AY170317.1 | Ehlers B., 2002 [28] |
| PLHV-3 (905) fw PLHV-3 (905) rev |
ACAAGAGCCTTAGGGTTCCAAACT GTGTCCAGTGTTGTAATGGATGCC |
13,472–13,495 13,727–13,704 |
AY170316.1 | Chmielewicz et al., 2003 [29] |
| PCV2 fw (F66) PCV2 rev (B67) |
GGTTTGTAGCCTCAGCCAAAGC GCACCTTCGGATATACTGTCAAGG |
567–546 152–175 |
KT868491.1 | Mankertz et al., 2000 [30] |
| PERV env C.2 fw PERV env C.2 rev |
GATTAGAACTGGAAGCCCCAAGTGCTCT TCTGATCCAGAAGTTATGTTAGAGGATGGT |
9362–9389 9649–9620 |
AM229312 | Dieckhoff et al., 2009 [26] |
| Primers and probes used to detect PERV-A/C | ||||
| PERV-A-VRBF PERV-C-TMR PERV-C rev |
CCTACCAGTTATAATCAATTTAATTATGGC C TCAAACCACCCTTGAGTAGTTTCC TATGTTAGAGGATGGTCCTGGTC |
Wood et al., 2004 [7] Dieckhoff et al., 2009 [26] |
||
| Primers and probes used for real-time PCR | ||||
| PCV3 fw PCV3 rev PCV3 probe |
AGTGCTCCCCATTGAACG ACACAGCCGTTACTTCAC FAM-ACCCCATGG-Zen-CTCAACACATATGACC-BHQ |
1427–1444 1561–1544 1473–1449 |
KT869077 | Palinski et al., 2016 [31] |
| pGAPDH fw pGAPDH rev pGAPDH probe |
GATCGAGTTGGGGCTGTGACT ACATGGCCTCCAAGGAGTAAGAHEX-CCACCAACCCCAGCAAGAGCACGC-BHQ |
1083–1104 1188–1168 1114–1137 |
NM_001206359.1 | Duvigneau et al., 2005 [32] |
| HEV fw HEV rev HEV probe |
GGTGGTTTCTGGGGTGAC AGGGGTTGGTTGGATGAA FAM-TGATTCTCAGCCCTTCGC-BHQ |
5261–5278 5330–5313 5284–5301 |
M73218. | Jothikumar et al., 2006 [17] |
| PCMV fw PCMV rev PCMV probe | ACTTCGTCGCAGCTCATCTGA GTTCTGGGATTCCGAGGTTG FAM-CAGGGCGGCGGTCGAGCTC-BHQ | Mueller et al., 2002 [33] | ||
| PERV pol fw PERV pol rev PERV pol probe |
CGACTGCCCCAAGGGTTCAA TCTCTCCTGCAAATCTGGGCC FAM-CACGTACTGGAGGAGGGTCACCTG-BHQ |
3568–3587 3803–3783 3655–3678 |
HM159246 | Yang et al., 2015 [21] |
| Primers used to detect spliced mRNA | ||||
| P1 P2 P3 |
TGCTGTTTGCATCAAGACCGC ACAGACACTCAGAACAGAGAC ATGGAGGCGAAGCTTAAGGGGA |
Karlas et al. [23] | ||
| Primers and probes used for dd PCR | ||||
| pGAPDH fw pGAPDH rev pGAPDH probe |
TTCACTCCGACCTTCACCA CCGCGATCTAATGTTCTCTTTC HEX-CAGCCGCGTCCCTGAGACAC-BHQ |
3951–3970 4022–4001 3991–3972 bp |
396823 | Yang et al., 2015 [21] |