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. 2020 Jan 16;12(1):112. doi: 10.3390/v12010112

Figure 3.

Figure 3

Loss of CD83 expression on/in HSV-2-infected mature DCs (mDCs). Mature DCs were mock-, HSV-1- (MOI of 2), or HSV-2-infected (MOI of 5) and treated with 10 µM MG-132 at 1 hpi, or DMSO as control. DCs were harvested 16 hpi and (A–C) subjected to flow cytometric or (D) Western blot analyses. (A–C) The cells were stained with specific fluorochrome-labeled antibodies directed against (A) CD83, (B) CD86, and (C) CD11c. Median values are shown as percentages relative to mock-treated DCs. The experiment was performed seven times with cells from different healthy donors. Error bars indicate ± SD. Significant changes (**** = p < 0.0001) are marked by asterisks. (D) Protein lysates were prepared and equal amounts were loaded on a 12% acrylamide SDS-gel, which was subjected to Western blotting. Protein levels of CD83, HSV-1/2 immediate early protein ICP0, and GAPDH were detected. Representative data out of four experiments are shown.