Estrogenic activities of E. bicolor latex extract and its phytochemicals in a steroid-regulated yeast system (BJ3505): (a) E. bicolor latex extract; (b) and its phytochemicals, coumestans (coumestrol) and diterpenes (abietic acid, resiniferatoxin (RTX)); (c) flavonoids (chalcone, kaempferol, quercetin, naringenin, quercetin, and rutin); (d) and isoflavones (biochanin A, daidzein, genistein). The average activities for positive controls estradiol and genistein were 2353.33 ± 166.6 and 998.9 ± 46.5 MU, respectively. The activity of yeast cultures without any treatments (negative control) ranged from 12.2 ± 0.8 to 24.8 ± 9.3 MU. Estrogenic activity of latex extract was analyzed by one-way ANOVA followed by Tukey’s posthoc test at p ≤ 0.05. Estrogenic activity of latex phytochemicals was analyzed by two-way ANOVA followed by Bonferroni posthoc test at p ≤ 0.05. Bars with asterisks for each group of chemicals tested are significantly different from each other. ns = non-significant.