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. 2019 Dec 25;12(1):59. doi: 10.3390/nu12010059

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antiestrogenic activities of E. bicolor latex extract and its phytochemicals in a steroid-regulated yeast system (BJ3505): (a) E. bicolor latex extract; (b) its phytochemicals coumestans (coumestrol) and diterpenes (abietic acid, RTX); (c) flavonoids (chalcone, kaempferol, quercetin, naringenin, quercetin, and rutin); (d) and isoflavones (biochanin A, daidzein, genistein). The average activity of estradiol (positive control) was 2353.33 ± 166.6 MU. The estrogenic activity of yeast cultures without any treatments (negative control) ranged from 12.2 ± 0.8 to 24.8 ± 9.3 MU. Antiestrogenic activity of latex extract was analyzed by one-way ANOVA followed by Tukey’s posthoc test at p ≤ 0.05. Antiestrogenic activity of latex phytochemicals were analyzed by two-way ANOVA followed by Bonferroni posthoc test at p ≤ 0.05. Bars with asterisks are significantly different from each other. ns = non-significant.