Skip to main content
. 2019 Dec 20;12(1):11. doi: 10.3390/v12010011

Figure 2.

Figure 2

Recombinant virus SDLY107-VP1. (A) The PCR product of the 1430–2459 bp upstream fragment of VP1 of the SDLY107 strain, the 3308–3742 bp downstream fragment, and the VP1 fragment of the SDJN2015-01 strain. M is DL2000 DNA Marker; Lane 1 is upstream 1430–2459 bp of VP1 of the SDLY107 strain, and the length is about 1029 bp; Lane 2 is VP1 fragment of the SDJN2015-01 strain, and the length is about 891 bp; Lane 3 is downstream 3308–3742 bp of VP1 of the SDLY107 strain, and the length is approximately 434 bp. (B) The fusion products of the 1430–2459 bp upstream fragment, 3308–3742 bp downstream fragment and VP1 fragment of the SDJN2015-01 strain were obtained. M is a DL5000 DNA Marker; Lanes 1–3 are three DNA fragment fusion PCR products, and the length is about 2313 bp. (C) Plasmid pMD-19T-107 and pMD-19T-VP1 double digestion enzyme electrophoresis. M is DL1,0000 DNA Marker; Lane 1 is the product of the pMD-19T-107 plasmid after Nru I and BsiW I digestion, the fragment length is about 8.1 kb and 2.0 kb; Lane 2 is the pMD-19T-VP1 plasmid after Nru I and BsiW I digestion. The product fragment length is approximately 3.0 kb and 2.0 kb. (D) Plasmid pMD-19T-107-VP1 double digestion enzyme electrophoresis. M is DL1,0000 DNA Marker; Lanes 1 and 2 are the product of the pMD-19T-107-VP1 plasmid digested with XbaI and HindIII, and the fragment length is about 7.4 kb and 2.7 kb.