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Representative images of immunohistochemistries for BMP2, CD31, CD68, CD63 and Alix on both calcified and non-calcified regions of aortic valve cusps. Images of a haematoxylin and eosin stain are shown to facilitate recognition of calcium deposits as acellular and eosinophilic areas with an amorphous extracellular matrix. LV = left ventricular side; Ao = aortic side.
