Figure 9.

CD8⁺ T cell function during SINV infection in WT, Ifngr1^−/−, and Ifng^−/− mouse brains and spinal cords. (A–D) Flow cytometry was used to evaluate the percentage of CD8⁺ T cells producing IFN-γ (A), TNF-α (B), GM-CSF (C), and granzyme B (D) in the brains of WT (black bars), Ifngr1^−/− (gray bars), and Ifng^−/− (white bars) mice at 7 DPI. (E) MFI presented in graph form (left) and as a histogram (right) was used to evaluate the amount of granzyme B produced by individual CD8⁺ T cells among strains (n = 3–9 pooled mice per strain per time point from 3–4 independent experiments; * p < 0.05, ** p < 0.01 by Dunn’s multiple comparisons test). (F–J) Relative mRNA expression of granzyme A (F), granzyme B (G), granzyme K (H), granzyme M (I), and perforin (J) were examined by qRT-PCR in WT (black circle and solid line), Ifngr1^−/− (gray square and solid line), and Ifng^−/− (white circle and black dashed line) mouse brains (n = 3–4 mice per strain per time point; data are presented as the mean ± SEM; dashed line indicates gene expression of 0 DPI tissue for each strain to which other time points were normalized; * p < 0.05, WT vs. Ifngr1^−/−; ^## p < 0.01, WT vs. Ifng^−/−, ^† p < 0.05, ^††† p < 0.001, Ifngr1^−/− vs. Ifng^−/−, all by Tukey’s multiple comparisons test). (K–N) Flow cytometry was used to evaluate the percentage of CD8⁺ T cells producing IFN-γ (K), TNF-α (L), GM-CSF (M), and granzyme B (N) in the spinal cords of WT, Ifngr1^−/−, and Ifng^−/− mice at 7 DPI. (n = 6–9 pooled mice per strain per time point from five independent experiments; * p < 0.05, by Dunn’s multiple comparisons test).