Figure 10.

Effect of IFN-γ on CD8⁺ T cell and NK cell degranulation and cytotoxicity. (A–D) Flow cytometry was used to examine the percentage of CD8⁺ T cells (A,C) or NK cells (B,D) expressing CD107a, indicative of degranulation, in CLNs (A,B) and brains (C,D) of WT (black bars), Ifngr1^−/− (gray bars), and Ifng^−/− (white bars) mice at 7 DPI (n = 6–9 pooled mice per time point from four independent experiments; data are presented as the mean ± SEM; * p < 0.05, **** p < 0.0001 by Dunn’s multiple comparisons test). (E,F) Contour plots (E) show the gating, denoted by blue lines, of CD8⁺ T cells (top row of plots) and NK cells (bottom row of plots) for granzyme A and granzyme B production in the brain at 7 DPI. Boolean gating (F) was used to determine whether stimulated CD8⁺ T cells (top row) and NK cells (bottom row) were producing both granzymes A and B (light gray), granzyme A only (dark gray), granzyme B only (white), or neither of the granzymes (black) at 7 DPI (n = 6–9 pooled mice per time point from three independent experiments; data are presented as the mean).