Figure 2.

Mutations conferring resistance to trans-dominant RevM10 therapy induce a conformational change in the HIV-1 RRE. (A) Structural probing of the wild-type RRE (left) and RevM10-resistant RRE-61 (right). (C) Control DNA sequencing lane from which nucleotide numbering was derived. Designations − and + refer to untreated and NMIA-treated RNA, respectively. Major alterations in chemical reactivity are indicated by the bar. (B,C) Cartoons depicting SHAPE-derived conformations of wild-type RRE and RRE-61. SL, stem-loop. Positions of RRE point mutations, inducing RevM10 resistance, are indicated by asterisks in (B). Modified from Reference [47].