Figure 7.

Analysis of G2AP particle RT content. (A) HEK 293T cells were transfected with wt or G2AP expression vectors. At 2–3 days post-transfection, supernatants were collected, filtered, and centrifuged through 20% sucrose cushions. Viral pellets from equal amounts of supernatants were resuspended, prepared, and subjected to Western immunoblotting. HIV-1 Gag and Pol proteins were respectively probed with anti-p24CA and anti-HIV-1 RT polyclonal antibodies. (B) Virus-associated RT levels. Quantities of p24gag-associated Gag (Pr55gag, p41gag and p24gag) and RT-associated Pol, Gag–Pol and p66/51RT in each sample were quantified using immunoblot scanning band densities. Ratios of total Pol to Gag protein levels were calculated for G2AP and normalized to those of a wt in parallel experiments. Data are from three independent experiments. Error bars indicate standard deviation. ** p < 0.01.