Table 1.
Virus titers of HIV-1 mutants.
| Construct a | Titer (c.f.u./mL) | Relative Titer | Mean Relative Titer | |
|---|---|---|---|---|
| Mutant | Dp6*PR b | (%) | (%) ± SD c | |
| D2APol | 9 | 5500 | 0.164 | |
| 11 | 5000 | 0.220 | ||
| 8 | 3750 | 0.213 | ||
| 11 | 5500 | 0.200 | 0.199 ± 0.025 | |
| D2A/P22APol | 11,000 | 5500 | 200.0 | |
| 11,000 | 5000 | 220.0 | ||
| 9000 | 3750 | 240.0 | ||
| 8750 | 5500 | 159.1 | 204.8 ± 34.56 | |
| D2A/D15EPol | 8250 | 5500 | 150.0 | |
| 10,500 | 5000 | 210.0 | ||
| 6250 | 3750 | 166.7 | ||
| 8250 | 5500 | 150.0 | 169.2 ± 28.33 | |
a Each construct was cotransfected with a VSV-G expression vector into 293T cells. At 48 h post-transfection, supernatants were collected, filtered, and used to infect HeLa cells. Infection and selection of drug-resistant colonies were performed as described in Materials and Methods. b Dp6*PR titers were determined in parallel experiments performed in quadruplicate. c Percentage of relative titers were determined by dividing the mutant titer by the Dp6*PR titer in parallel experiments and multiplying by 100%. Mean and standard deviation (SD) values are indicated.