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. 2020 Jan 16;12(1):114. doi: 10.3390/v12010114

Table 1.

Primers used for the quantitative real-time polymerase chain reaction (qRT-PCR).

Target Primer Sequence (5′–3′) Gen Bank Accession No. Reference
GAPDH Forward GAACATCATCCCAGCGTCCA NM_204305.1 [16]
Reverse CGGCAGGTCAGGTCAACAAC
IFN-α Forward GACAGCCAACGCCAAAGC GU119896.1 [16]
Reverse GTCGCTGCTGTCCAAGCATT
IFN-β Forward GCCCACACACTCCAAAACACTG NM_001024836.1 [16]
Reverse TTGATGCTGAGGTGAGCGTTG
IL-1β Forward GGTCAACATCGCCACCTACA NM_204524.1 [16]
Reverse CATACGAGATGGAAACCAGCAA
TNF-α Forward GCTGTTCTATGACCGCCCAGTT NM_204267.1 [16]
Reverse AACAACCAGCTATGCACCCCA
IL-2 Forward GCTAATGACTACAGCTTATGGAGCA AF000631.1 [16]
Reverse TGGGTCTCAGTTGGTGTGTAGAG
IL-6 Forward AAATCCCTCCTCGCCAATCT AJ309540.1 [16]
Reverse CCCTCACGGTCTTCTCCATAAA
IL-10 Forward AGCAGATCAAGGAGACGTTC NM_001004414.2 [16]
Reverse ATCAGCAGGTACTCCTCGAT
TLR3 Forward ACAATGGCAGATTGTAGTCACCT NM_001011691.3 [16]
Reverse GCACAATCCTGGTTTCAGTTTAG
TLR7 Forward TCTGGACTTCTCTAACAACA NM_001011688.2 [16]
Reverse AATCTCATTCTCATTCATCATCA
MHC-I Forward AAGAAGGGGAAGGGCTACAA NM_001031338.1 [16]
Reverse AAGCAGTGCAGGCAAAGAAT
MHC-II Forward CTCGAGGTCATGATCAGCAA DQ008588.2 [16]
Reverse TGTAAACGTCTCCCCTTTGG
IFN-γ Forward CCTCCAACACCTCTTCAACATG X92479 [17]
Reverse TGGCGTGCGGTCAAT
IL-4 Forward TCGAGGAGTGACGGGTG AJ621249.1 [17]
Reverse ACTATCCGGATGCTCTCCATC
IL-5 Forward GGAACGGCACTGTTGAAAAATAA AJ621252.1 [18]
Reverse TTCTCCCTCTCCTGTCAGTTGTG
IL-13 Forward CTGCCCTTGCTCTCCTCTGT AJ621250.1 [18]
Reverse CCTGCACTCCTCTGTTGAGCTT
Granzyme A Forward ACTCATGTCGAGGGGATTCA NM_204457.1 [19]
Reverse TGTAGACACCAGGACCACCA
Granzyme K Forward CGGGAAGCAACTGTTGAAAT XM_423832 [19]
Reverse GAGTCTCCCTTGCAAGCATC
MDA5 Forward GGACGACCACGATCTCTGTGT NM_001193638.1 [20]
Reverse CACCTGTCTGGTCTGCATGTTATC
CXCLi1 Forward AACTCCGATGCCAGTG NM_205018.1 [21]
Reverse TTGGTGTCTGCCTTGT
CXCLi2 Forward CATCATGAAGCATTCCATCT NM_205498.1 [21]
Reverse CTTCCAAGGGATCTTCATTT
TGF-β3 Forward TCTTTACATTGACTTCCGAC NM_205454.1 [21]
Reverse TCCTCCCAACATAGTACAAG
MX1 Forward AAGCCTGAGCATGAGCAGAA NM_204609.1 [22]
Reverse TCTCAGGCTGTCAACAAGATCAA
OASL Forward AGATGTTGAAGCCGAAGTACCC NM_205041.1 [22]
Reverse CTGAAGTCCTCCCTGCCTGT
ISG12-2 Forward TCAATGGGTGGCAAAGGAG NM_001001296.5 [22]
Reverse TACAGGGAGAGCAAAGAAGAGAAGA
IFIT5 Forward CAGAATTTAATGCCGGCTATGC XM_421662.4 [22]
Reverse TGCAAGTAAAGCCAAAAGATAAGTGT
USP18 Forward CAACGTGGGAAGAGGAGAAA XM_416398.3 [22]
Reverse ACTTCATGAGCGGAGAAGGA
IRF3/7 Forward ACTGACCAGCCCAGGAACTCT NM_205372.1 [22]
Reverse AAGGCTTTCCCAACCACAAA
SST Forward GGTCCACGGTTATGGTGAAAG NM_205336.1 [22]
Reverse GGTCAGAAATCACAACTCAAGCA
KHSRP Forward CAGCGGGGAAATGATTAAGAAG NM_204277.1 [22]
Reverse TTTGTGTGTGGGGATGGAGA
Perforin Forward ATGGCGCAGGTGACAGTGA XM_425355 [23]
Reverse TGGCCTGCACCGGTAATTC
PARP Forward ATTGTGGAGGAGCTGGGAGGAA NM_205263 [23]
Reverse AGGCTTGCTGCACTTCCCATC
HMG-2 Forward AGAGCACAAGAAGAAGCAC M80574 [23]
Reverse GTCTTTTAGGAGCGTTGGGGTC
NK lysin Forward GATGGTTCAGCTGCGTGGGATGC DQ186291 [23]
Reverse CTGCCGGAGCTTCTTCAACA