Table 1.
Target | Primer | Sequence (5′–3′) | Gen Bank Accession No. | Reference |
---|---|---|---|---|
GAPDH | Forward | GAACATCATCCCAGCGTCCA | NM_204305.1 | [16] |
Reverse | CGGCAGGTCAGGTCAACAAC | |||
IFN-α | Forward | GACAGCCAACGCCAAAGC | GU119896.1 | [16] |
Reverse | GTCGCTGCTGTCCAAGCATT | |||
IFN-β | Forward | GCCCACACACTCCAAAACACTG | NM_001024836.1 | [16] |
Reverse | TTGATGCTGAGGTGAGCGTTG | |||
IL-1β | Forward | GGTCAACATCGCCACCTACA | NM_204524.1 | [16] |
Reverse | CATACGAGATGGAAACCAGCAA | |||
TNF-α | Forward | GCTGTTCTATGACCGCCCAGTT | NM_204267.1 | [16] |
Reverse | AACAACCAGCTATGCACCCCA | |||
IL-2 | Forward | GCTAATGACTACAGCTTATGGAGCA | AF000631.1 | [16] |
Reverse | TGGGTCTCAGTTGGTGTGTAGAG | |||
IL-6 | Forward | AAATCCCTCCTCGCCAATCT | AJ309540.1 | [16] |
Reverse | CCCTCACGGTCTTCTCCATAAA | |||
IL-10 | Forward | AGCAGATCAAGGAGACGTTC | NM_001004414.2 | [16] |
Reverse | ATCAGCAGGTACTCCTCGAT | |||
TLR3 | Forward | ACAATGGCAGATTGTAGTCACCT | NM_001011691.3 | [16] |
Reverse | GCACAATCCTGGTTTCAGTTTAG | |||
TLR7 | Forward | TCTGGACTTCTCTAACAACA | NM_001011688.2 | [16] |
Reverse | AATCTCATTCTCATTCATCATCA | |||
MHC-I | Forward | AAGAAGGGGAAGGGCTACAA | NM_001031338.1 | [16] |
Reverse | AAGCAGTGCAGGCAAAGAAT | |||
MHC-II | Forward | CTCGAGGTCATGATCAGCAA | DQ008588.2 | [16] |
Reverse | TGTAAACGTCTCCCCTTTGG | |||
IFN-γ | Forward | CCTCCAACACCTCTTCAACATG | X92479 | [17] |
Reverse | TGGCGTGCGGTCAAT | |||
IL-4 | Forward | TCGAGGAGTGACGGGTG | AJ621249.1 | [17] |
Reverse | ACTATCCGGATGCTCTCCATC | |||
IL-5 | Forward | GGAACGGCACTGTTGAAAAATAA | AJ621252.1 | [18] |
Reverse | TTCTCCCTCTCCTGTCAGTTGTG | |||
IL-13 | Forward | CTGCCCTTGCTCTCCTCTGT | AJ621250.1 | [18] |
Reverse | CCTGCACTCCTCTGTTGAGCTT | |||
Granzyme A | Forward | ACTCATGTCGAGGGGATTCA | NM_204457.1 | [19] |
Reverse | TGTAGACACCAGGACCACCA | |||
Granzyme K | Forward | CGGGAAGCAACTGTTGAAAT | XM_423832 | [19] |
Reverse | GAGTCTCCCTTGCAAGCATC | |||
MDA5 | Forward | GGACGACCACGATCTCTGTGT | NM_001193638.1 | [20] |
Reverse | CACCTGTCTGGTCTGCATGTTATC | |||
CXCLi1 | Forward | AACTCCGATGCCAGTG | NM_205018.1 | [21] |
Reverse | TTGGTGTCTGCCTTGT | |||
CXCLi2 | Forward | CATCATGAAGCATTCCATCT | NM_205498.1 | [21] |
Reverse | CTTCCAAGGGATCTTCATTT | |||
TGF-β3 | Forward | TCTTTACATTGACTTCCGAC | NM_205454.1 | [21] |
Reverse | TCCTCCCAACATAGTACAAG | |||
MX1 | Forward | AAGCCTGAGCATGAGCAGAA | NM_204609.1 | [22] |
Reverse | TCTCAGGCTGTCAACAAGATCAA | |||
OASL | Forward | AGATGTTGAAGCCGAAGTACCC | NM_205041.1 | [22] |
Reverse | CTGAAGTCCTCCCTGCCTGT | |||
ISG12-2 | Forward | TCAATGGGTGGCAAAGGAG | NM_001001296.5 | [22] |
Reverse | TACAGGGAGAGCAAAGAAGAGAAGA | |||
IFIT5 | Forward | CAGAATTTAATGCCGGCTATGC | XM_421662.4 | [22] |
Reverse | TGCAAGTAAAGCCAAAAGATAAGTGT | |||
USP18 | Forward | CAACGTGGGAAGAGGAGAAA | XM_416398.3 | [22] |
Reverse | ACTTCATGAGCGGAGAAGGA | |||
IRF3/7 | Forward | ACTGACCAGCCCAGGAACTCT | NM_205372.1 | [22] |
Reverse | AAGGCTTTCCCAACCACAAA | |||
SST | Forward | GGTCCACGGTTATGGTGAAAG | NM_205336.1 | [22] |
Reverse | GGTCAGAAATCACAACTCAAGCA | |||
KHSRP | Forward | CAGCGGGGAAATGATTAAGAAG | NM_204277.1 | [22] |
Reverse | TTTGTGTGTGGGGATGGAGA | |||
Perforin | Forward | ATGGCGCAGGTGACAGTGA | XM_425355 | [23] |
Reverse | TGGCCTGCACCGGTAATTC | |||
PARP | Forward | ATTGTGGAGGAGCTGGGAGGAA | NM_205263 | [23] |
Reverse | AGGCTTGCTGCACTTCCCATC | |||
HMG-2 | Forward | AGAGCACAAGAAGAAGCAC | M80574 | [23] |
Reverse | GTCTTTTAGGAGCGTTGGGGTC | |||
NK lysin | Forward | GATGGTTCAGCTGCGTGGGATGC | DQ186291 | [23] |
Reverse | CTGCCGGAGCTTCTTCAACA |