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. 2020 Jan 19;9(1):278. doi: 10.3390/jcm9010278

Figure 3.

Figure 3

Intracellular cytokine ratios of alveolar macrophages (AMs) suggest a change from M1 (pro-inflammatory) to M2 (anti-inflammatory) status after co-culture with bone marrow stromal cells (MSCs). (A) Bronchoalveolar lavage (BAL) cells are depicted in red, BAL cells from co-cultures are pictured in blue. The co-cultured BAL cells demonstrate a clear shift towards the right, indicating an increase in the mean fluorescent intensity (MFI) of the IL-10+ population, and upwards, indicating an increase in the MFI of CD206 in these cells. Due to the lower percentage of cells producing TNF-α, a leftward shift is not as evident, although an upward shift indicating increased MFI of CD206 in these cells is present. (B) The fold change of the ratio of IL-10 to TNF-α for the BAL–MSC co-culture is shown for both subjects (sarcoidosis subjects 16 and 17). BAL cells cultured alone are depicted by the red bars (the ratio was converted to a value of 1), and co-cultured BAL cells are depicted by the blue bars (representing the fold increase of the ratio). The bar graph shows that—although not to the same extent—the BAL cells shifted towards a more anti-inflammatory state in both subjects.