Figure 3.

Altering the stability of the WNV 3′SL affects RNA replication differently in human and mosquito cells. (A) Huh7 cells were transfected with wild-type and mutant WNVRluc replicon RNAs, cultivated at 37 °C and analyzed for luciferase reporter activity at the indicated time points post transfection. Results from one representative experiment performed in triplicate are shown and error bars reflect standard deviations. (B) Same as in (A) except that C6/36 cells were used and cultivated at 28 °C.