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. 2020 Jan 15;12(1):104. doi: 10.3390/v12010104

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The cultivation temperature determines the replication rates of WNVRluc replicons. (A) Huh7 cells were transfected with wild-type and mutant WNVRluc replicon RNAs, subsequently cultivated at 28 °C and analyzed for luciferase reporter activity at the indicated time points post transfection. Results from one representative experiment performed in triplicate are shown and error bars reflect standard deviations. (B) Same as in (A) except that C6/36 cells were used and cultivated at 37 °C after transfection.