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. 2020 Jan 15;12(1):104. doi: 10.3390/v12010104

Figure 5.

Figure 5

Mosquito cells encode two AUF1-homologous proteins. (A) Alignment of human AUF1 p45 and A. albopictus proteins p30 and p32. Conserved RNP-1 (light green) and RNP-2 (green) sequences of the RNA recognition motifs (RRM) are highlighted. The arginine residues of the RGG/RG motif of AUF1 p45 that were found to be dimethylated in human cells are highlighted in blue. The alternatively spliced sequence that is absent in p30 but present in p32 is shown in red. (B) Domain organization of human AUF1 p45 and mosquito squid proteins p30 and p32. (C) AUF1-homologous proteins p30 and p32 were produced in, and purified from, E. coli. About 4 μg of protein were analyzed on a Coomassie-stained SDS-gel in parallel with a molecular weight marker (M).