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The effect of temperature on rates of RNA chaperoning and RNA annealing reactions. The RNA chaperoning (left) and RNA annealing (right) reactions in the presence of AUF1 p45 (A), p32 (B), or p30 (C) were performed at 22 °C and 28 °C. The rate constants were normalized by subtracting the non-enzymatic rate from the total rate and plotted as a function of the protein concentration.
