Figure 3.

The oBRB model prototype built using the proposed device. (A) Schematic showing the anatomical structure of oBRB (retinal pigment epithelial cells–Bruch membrane-choroids). (B–E) tri-culture of ARPE–human umbilical vein endothelial cells (HUVEC)-human lung fibroblasts (NHLF). (B) ARPE cells stained in red were cultured in the upper channel. (C) GFP-HUVEC microvessels were cultured in the lower channel. (D) Overlay of (B) and (C). (E) Confocal microscopic view of the cross-section of the co-culture model.