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. 2019 Dec 30;11(1):50. doi: 10.3390/mi11010050

Figure 6.

Figure 6

Immunohistochemistry analysis, calcium deposition, and proteoglycan formations of the osteogenic and chondrogenic specific markers in hAMSCs seeded into microfluidics channels containing fibrin scaffolds with and without AuNWs. The differentiated hAMSCs stained by collagen II and toluidine blue characterize the chondrogenic lineage, and collagen I antibody and alizarin red characterize the osteogenic lineage. Differentiated hAMSCs seeded on different substrates (see Table 1): (A) differentiated hAMSCs stained with collagen II antibody, (B) differentiated hAMSCs stained with collagen I antibody, (C) the proteoglycans formation stained with toluidine blue and the differentiated hAMSCs, and (D) differentiated hAMSCs seeded on microfluidics substrates stained with alizarin red. Scale bar for rows number 1–3 = 30 µm and for row number 4 = 50 µm.