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. 2020 Jan 13;12(1):201. doi: 10.3390/nu12010201

Figure 9.

Figure 9

Silk peptide inhibits fat accumulation and increases browning in primary cultures of subcutaneous white adipocytes. (A) The viability of primary adipocytes treated with SP for 24 h was assessed using an MTT assay. (B) The effect of SP on lipid accumulation was assessed using Oil red O staining. (C, D) The protein expression levels of C/EBPα, FABP4, CPT1, and UCP1 were measured in subcutaneous white adipocytes. (E) Immunofluorescence images of adipocytes were captured at 800× magnification. After differentiation, cells were fixed with methanol and then stained with MitoTracker® 363 Deep Red or anti-UCP1 antibody. (F) Browning was promoted in adipocytes cultured with 50 nM T3 and 1 mM rosiglitazone and treated with SP, and the protein expression of BAT-specific genes was measured. Data are expressed as mean ± SD (n = 4). Values with different letters were significantly different; p < 0.05 (a > b > c > d > e).