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. 2020 Jan 7;12(1):165. doi: 10.3390/nu12010165

Figure 3.

Figure 3

The effects of NCM 1921 on the production of Th2 and Th1 cytokines (IFN-g) by cultured splenocytes in NC/Nga mice. The levels of (A) IL-4, (B) IFN-γ, (C) IL-5, and (D) IL-13 in the cell culture supernatant were measured by ELISA. Splenocytes were cultured for 48 h at a concentration of 1 × 105 cells/well by using anti-CD3 antibody–coated 96-well plates at 15 weeks of age after atopic dermatitis (AD) induction using DNCB and subsequent treatment with NCM 1921 or dexamethasone. The non-coated cells represent the negative control (i.e., no exposure to the anti-CD3 antibody). NC/Nga_Nor: normal control; DNCB_CTL: 1-chloro-2,4-dinitrobenzene (DNCB)-treated control; DNCB_Dexa 3 mg/kg: 3 mg/kg dexamethasone; DNCB_NCM 1921 300 mg/kg: 300 mg/kg NCM 1921. Values are expressed as the means ± SEMs (n = 6). ## p < 0.01 and ### p < 0.001 compared with NC/Nga_Nor; * p < 0.05 and *** p < 0.001 compared with DNCB_CTL, as determined by ANOVA followed by multiple comparison tests.