Table 1.
Fluorescence-activated cell sorting analysis (FACS) of immune cell subtypes in the axillary lymph nodes (ALNs), spleens, and dorsal skin tissues of DNCB-atopy dermatitis murine models.
| Cell Phenotypes | DNCB | ||||
|---|---|---|---|---|---|
| NC/Nga_Nor | CTL | Dexa_3 mg/kg | NCM1921_300 mg/kg | ||
| WBC | Total WBCs cells (×103/µL) | 3.6 ± 0.5 | 1.7 ± 0.1 ## | 3.3 ± 0.5 ** | 3.1 ± 0.5 ** |
| WBCs differential counting (%) | |||||
| Neutrophils | 16.7 ± 1.4 | 36.7 ± 2.7 | 25.8 ± 0.7 | 25.4 ± 2.2 | |
| Lymphocytes | 79.1 ± 1.1 | 58.5 ± 2.3 | 70.3 ± 0.6 | 69.5 ± 2.3 | |
| Monocytes | 0.48 ± 0.1 | 0.43 ± 0.1 | 0.65 ± 0.1 | 0.40 ± 0.0 | |
| Eosinophils | 1.28 ± 0.3 | 1.17 ± 0.2 | 1.65 ± 0.2 | 2.58 ± 0.5 | |
| Basophils | 0.28 ± 0.1 | 0.33 ± 0.0 | 0.33 ± 0.0 | 0.25 ± 0.1 | |
| ALN | Total ALN cells (×104/mL) | 5.3 ± 1.6 | 184. ± 22.5 | 38.3 ± 12.2 | 104.3 ± 10.9 |
| CD3+/CD19− (×105 Cells) | 0.93 ± 0.27 | 30.9 ± 4.57 ### | 6.54 ± 2.11 *** | 30.2 ± 5.85 | |
| CD4+/CD8+ (×105 Cells) | 0.66 ± 0.13 | 22.2 ± 2.85 ### | 6.28 ± 1.87 *** | 19.5 ± 3.95 | |
| CD4+/CD69+ (×105 Cells) | 0.59 ± 0.15 | 7.50 ± 1.42 ### | 1.65 ± 0.52 ** | 5.84 ± 1.14 | |
| CD23+/B220+ (×105 Cells) | 0.95 ± 0.24 | 130.2 ± 19.8 ### | 27.0 ± 10.2 *** | 62.6 ± 7.95 ** | |
| Spleen | Total spleen cells (×104/mL) | 665.0 ± 99.1 | 1585.0 ± 231.0 | 810.0 ± 144.0 | 850.0 ± 103.0 |
| CD3+/CD19− (×105 Cells) | 265.4 ± 35.8 | 561.8 ± 136.2 # | 372.5 ± 71.7 | 3720 ± 41.7 | |
| CD4+/CD8+ (× 105 Cells) | 119.2 ± 25.5 | 226.0 ± 34.5 # | 162.7 ± 30.7 | 154.9 ± 25.6 | |
| CD4+/CD69+ (×105 Cells) | 7.52 ± 1.33 | 20.9 ± 7.3 | 13.7 ± 4.74 | 11.9 ± 1.96 | |
| CD23+/B220+ (×105 Cells) | 248.5 ± 80.7 | 509.36 ± 110.0 # | 294. ± 69.3 | 399.4 ± 71.4 | |
| Dorsal skin | Total dorsal skin cells (×104/mL) | 3.5 ± 0.5 | 27.5 ± 2.5 | 9.0 ± 1.0 | 10.5 ± 2.5 |
| CD3+(×105 Cells) | 0.2 ± 0.12 | 3.03 ± 0.28 ### | 1.53 ± 0.39 ** | 2.42 ± 0.01 * | |
| CCR3+/CD11b+ (×105 Cells) | 0.89 ± 0.06 | 3.79 ± 1.12 ## | 2.07 ± 0.03 | 1.69 ± 0.27 | |
Each point represents mean ± SEM values for six mice. # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with NC/Nga_Nor; * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with DNCB-CTL, as determined by one-way analysis of variance (ANOVA) followed by multiple comparison tests.