Figure 1.

(a) Scheme of the tripartite system to produce pseudoviruses, showing the trans-packaging constructs encoding C or C-Ca (numbered 1–3) and the Ca-PrME and the WNV replicon (with EGFP reporter). (b) Representative cytofluorimetry (EGFP) of VERO cells infected with the pseudoviruses produced with the indicated capsid constructs. (c) Quantification of data shown in (b) (N = 7). (d) Quantification of viral genomes packaged in the indicated pseudovirus preparations (N = 5) (e) Western blot of cell extracts and pellet supernatants of cells producing the indicated pseudoviruses, developed with mAb 4G2 (anti-E). β-actin was used as the loading control.