. Author manuscript; available in PMC: 2020 Feb 14.

Published in final edited form as: J Biomol NMR. 2019 Apr 30;73(3-4):191–198. doi: 10.1007/s10858-019-00245-5

Table 1.

Field-induced RDCs measured for the Robo1 Dy³⁺ and Tb³⁺ complexes at 900 MHz and 600 MHz. RDCs were measured as the difference in J+D in the paramagnetic complex and an average value of J from the diamagnetic complex

	Dy³⁺ Complex			Tb³⁺ Complex
Peak Label	⁹⁰⁰DCH	⁶⁰⁰DCH	Scaled	⁹⁰⁰DCH	⁶⁰⁰DCH	Scaled
3	−1.8 ± 1.3	1.4 ± 1.8	3.1 ± 3.6	1.4 ± 1.3	0.2± 1.9	0.4 ± 2.8
5	N/A^a	N/A^a	N/A^a	1.8 ± 1.8	0.8 ± 2.3	1.8 ± 3.4
6	0.1 ± 1.6	1.1 ± 1.5	2.5 ± 2.3	−0.3 ± 1.6	3.5 ± 2.3	7.9 ± 3.4
8	2.1 ± 1.8	1.1 ± 1.9	2.5 ± 2.3	5.4 ± 1.3	3.9 ± 0.9	8.8 ± 1.4
10	N/A^b	N/A^b	N/A^b	6.4 ± 1.7	N/A^b	N/A^b
12	6.2 ± 3.4	0.8 ± 2.5	1.8 ± 3.8	2.6 ± 1.8	N/A^b	N/A^b
15	N/A^a	N/A^a	N/A^a	4.1 ± 2.3	3.5 ± 1.6	7.9 ± 2.4
16	6.4 ± 1.6	3.5 ± 1.4	7.9 ± 2.0	7.1 ± 2.4	6.1 ± 1.6	13.7 ± 2.5
19	0.0 ± 0.1	−0.1 ± 0.2	−0.2 ± 0.3	−0.2 ± 0.2	0.2 ± 0.6	0.4 ± 0.8
25	−2.7 ± 1.8	2.2 ± 1.4	4.9 ± 2.0	2.0 ± 3.2	0.6 ± 2.6	1.3 ± 3.7
28	3.9 ± 1.1	2.5 ± 1.5	5.6 ± 2.3	4.4 ± 0.8	2.9 ± 1.6	6.5 ± 2.3
33	0.0 ± 0.1	0.0 ± 0.3	0.0 ± 0.4	−0.2 ± 0.1	−0.3 ± 0.2	−0.7 ± 0.3
36	2.7 ± 2.3	1.7 ± 2.5	3.8 ± 3.7	1.4 ± 2.1	−1.2 ± 2.4	−2.7 ± 3.5

Peaks were greatly attenuated and reliable measurements could not be made.

Peaks 10and 12 show significant overlap and could not be fit well in all spectra.. Errors are standard deviations propagated from curve fitting estimates.