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Amplification program for real-time polymerase chain reaction assay.
| No. | Step | Temperature | Time |
|---|---|---|---|
| 1. | Reverse transcriptase | 50°C | 20 min |
| 2. | Initial denaturation | 95°C | 15 min |
| 3. | Denaturation | 94°C | 45 s |
| 4. | Annealing | 60°C | 45 s |
The cycling conditions for the APMV-1 matrix primers consisted of 40 cycles
