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Frame sequence obtained from captured video demonstrating rotation of a trapped single micro-tool. Frames were extracted every 1.5 s. Red crosses in frame at 0 s mark immobilization trap positions. Yellow crosses mark positions of displaced microspheres. The solid and dotted yellow lines shown in frames for 1.5, 6, and 10.5 s reveal variance of one of the dimensions of projection of the microtool captured by camera.
