Figure 4.

Expression profile of SlCaCA superfamily genes in various organs containing fruit from 10 days after flowering (DAF) to ripeness. YL and ML represent young and mature leaves, respectively. (A) In order to accurately compare the expression levels between genes, the copy number corresponding to the amount of each mRNA was determined using the standard curve. The expression level was expressed as log₂, and the heat map was prepared by subtracting the expression level of each gene from the median; red represents the highest expression levels, white indicates median expression, and blue shows the lowest expression levels. Gray represents undetectable expression. Each log₂ expression value was based on the mean of three biological replicates. (B) To compare the expression levels among organs for each gene, expression levels were normalized in each gene. Color bar denotes Z-score: red represents the highest expression levels, white indicates mean expression, and blue shows the lowest expression levels in each gene. Gray represents undetectable expression. Hierarchical clustering was performed using Ward’s method. Each expression value was based on the mean of three biological replicates.